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Tuesday, September 30, 2014

Cost-effective and easy to handle biotin removal

Biotin is often present in cell culture media. Its nearly irreversible binding to Strep-Tactin has to be taken into account for two reasons when recombinant proteins secreted to the cell culture supernatant are aimed to be directly purified via Strep-Tactin affinity chromatography:
  • It efficiently competes with the (Twin-)Strep-tag target proteinthereby preventing it from getting bound to the resin
  • it inactivates Strep-Tactin resins and thus make a re-use impossible.
BioLock is a ready-to-use and cost effective solution for the removal of free biotin from cell culture supernatant. It simply has to be added to the cell culture supernatant prior to purification.
The required amount of BioLock for effective biotin blocking can easily be determined since 1ml BioLock blocks 70 μg biotin. In addition we provide a table with the required BioLock amounts for standard mammalian and insect cell culture media. More information (pdf 1.1MB)

For detailed product information please download the data sheet.

For additional information contact info@stratech.co.uk.


Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.

50% Discount Off Calcium Indicator Cal-520

50% discount off all pack sizes and formats of Cal-520


Cal-520™ (includes Cal-520™ and Cal-520FF™) provides the most robust homogeneous fluorescence-based assay tool for detecting intracellular calcium mobilization. Cal-520™ AM is a new fluorogenic calcium-sensitive dye with a significantly improved signal to background ratio and intracellular retention compared to the existing green calcium indicators (such as Fluo-3 AM and Fluo-4 AM). The higher signal/background ratio and better intracellular retention make the Cal-520™ calcium assay a robust tool for evaluating GPCR and calcium channel targets as well as for screening their agonists and antagonists.  Further information of Cal-502™ visit our dedicated webpage.

Offer valid until 31st December 2014. 


Promotion may not be used in conjunction with any other offer, please quote promtional code  CAL520_50 when ordering. Offer valid (UK customers only) for more than one purchase per customer, but may not be used on bulk orders. 


Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.

Watch Alpco Diagnistic's "Identification of Antibodies Directed Against Human Islet Cell Surface Molecules" Webinar

During this webinar, Dr.Phil Streeter of Oregon Health Science University presents an overview of a new set of monoclonal antibody reagents used to differentiate and isolate functionally distinct cells within the pancreas. The presentation also introduces the application of these antibodies in Flow Cytometry and Immunohistochemistry with the aim to detect pancreatic Acinar Ductal, Alpha and Beta Cell Populations.

Links:
Watch the recorded webinar
Download the webinar slides