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Wednesday, April 22, 2015

NEW Product: AAVanced™ Concentration Reagent – One-step rAAV particle isolationsys

Easiest method for rAAV isolation
  • Easy to use, single reagent
  • Isolate rAAV directly from media
  • No cell lysates or centrifugation
  • Saves time and cost-effective
  • Compatible with all serotypes

AAVanced™ Concentration Reagent

One-step rAAV particle isolation from packaging cell media
Traditional rAAV production methods requires multiple steps, including cell lysis and CsCl2 ultra-high-speed density gradient centrifugation, chromatography, or binding to affinity matrix columns. These are difficult to setup, time-consuming, and require specialized equipment for isolation of high-purity rAAV for in vivo experiments. As many studies have shown that different rAAV serotypes are efficiently secreted into the culture medium of transfected 293T cells during rAAV packaging, SBI has developed a novel reagent to facilitate fast, high-quality rAAV isolation.



Easiest Protocol to Isolate rAAV Particles

SBI has developed an innovative solution called AAVanced Concentration Reagent – a simple, one step rAAV concentration reagent for the isolation of rAAV particles from media. AAVanced Concentration Reagent is based on a proven nanoparticle technology and is specifically optimized for the precipitation of most AAV serotypes from culture medium. The rAAV virus produced using AAVanced Concentration Reagent has been tested successfully for in vitro and in vivo applications for multiple serotypes of AAV with no observed cytotoxic effects, which provides direct evidence for the utility of this reagent for demanding applications using rAAV. SBI’s AAVanced Concentration Reagent significantly reduces the complexity and time required for rAAV virus production, allowing researchers to focus on their research experiments rather than virus production.

AAVanced Concentration Reagent Produces Robust Virions

How does AAVance virus preps compare to tranditonal cell lysate preps?
To test this comparison, recombinant rAAV was packaged with a PGK-GFP expression shuttle vector. The rAAV particles were isolated either by the traditional cell lysis freeze-thaw with centifugation method or with the AAVanced Concentration Reagent from the packaging cell media. Equal amounts of rAAV were then added to HEK293 cells to test for infectivity based on GFP expression. Representative images of GFP expressing cells 10 days after infection.

AAVanced virus produces infectious virus

AAVanced Concentration Reagent

AAVanced Concentration Reagent (100 ml) 100 ml AAV100A-1
AAVanced Concentration Reagent (250 ml) 250 ml AAV110A-1

Online Resources

Where can I find more information about SBI?

Visit the manufacturer page at www.stratech.co.uk/SBI, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.

NEW Product: ViaCheck™ 25% Viability Control

Stratech Scientific are pleased to announce an addition to the Bangs Laboratories line of ViaCheck products for QC of automated viability analyzers such as the Vi-CELL XR.  The new ViaCheck 25% Viability Control.

Inventory will be available soon please contact info@stratech.co.uk for further information.

Catalog Code Description Volume (mL) Product Data Sheet Material Safety Data Sheet
VC25B ViaCheck™ 25% Viability Control 20 PDS 706 MSDS VC Viability Control

Comparison Study of the Vi-CELL® XR Cell Viability Analyzer and Z2™ Coulter Counter  K.Kilbride, M. Meza, R. Anglea, CYTO 2014, FT. Lauderdale, FL, May 17-21, 2014

Where can I find more information about Bangs Laboratories?

Visit the manufacturer page at www.stratech.co.uk/bangs_laboratories, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.

Tuesday, April 21, 2015

Product Focus: Biotium CF Dyes™ Range

CF™ dyes are a series of highly water-soluble fluorescent dyes spanning the visible and near-infrared (NIR) spectrum for labeling biomolecules, especially proteins and nucleic acids. Developed by scientists at Biotium using new breakthrough chemistries, CF™ dyes rival or exceed the quality of other commercial dyes, such as Alexa Fluor® dyes, due to the following novel features.

New rhodamine chemistry

Rhodamine dyes are known for their excellent photostability and good fluorescence quantum yield; consequently several of the Alexa Fluor® dyes bear the rhodamine core structure.

Unfortunately, traditional rhodamine chemistry makes it difficult to extend the fluorescence wavelength to the far-red region and even more challenging in the near-IR region, especially for water-soluble dyes for bioconjugation. Recently, Biotium scientists discovered a new way to prepare novel rhodamine dyes of any fluorescence color from green to near-IR.

The new chemistry is a key element in the development of many of our CF™ dyes, particularly our far-red CF™ dyes, which are not only bright and water-soluble but also extremely photostable.

Excellent labeling efficiency

Reactive dyes for bioconjugation are generally susceptible to hydrolysis, which can cause problems for shipping, handling and storage and result in lower labeling efficiency. Heavily sulfonated dyes, such as the Alexa Fluor® dyes, DyLight® dyes and IRDye® are particularly hygroscopic, worsening the hydrolysis problem.

For example, the percent of active Alexa Fluor® 488 succinimidyl ester (SE) could be well below 50% by the time of application (Alexa Fluor 488 microscale labeling kit product information sheet, Invitrogen). In contrast, all of Biotium’s amine-reactive CF™ dyes have a relatively stable form of SE, which is more resistant to hydrolysis than the SE in many of the Alexa Fluor dyes. Accordingly, CF™ dye SE products generally give consistently higher labeling efficiency, thus providing users a better value.

Multi-color flexibility

Biotium currently offers 20 CF™ dyes spanning the visible, far-red, and near-infrared spectra, with additional colors in development. The CF™ dye product line includes reactive dyes, secondary antibodies, streptavidin, phallodin and other conjugates, and antibody labeling kits.

Unrivaled near-IR dyes

Near-IR dyes are typically much larger in size than dyes in the visible range. The large size often results in serious problems of low dye solubility, dye aggregation and poor fluorescence quantum yield. To overcome the problems, many commercial near-IR dyes, such as the near-IR Alexa Fluor® dyes, DyLight® dyes and IRDyes®, are prepared by placing a number of negatively charged sulfonate group on the dyes. While sulfonation improves dye solubility and fluorescence quantum yield to some degree, it creates another even more serious problem: non-specific binding of the bioconjugates prepared from the dyes.

For example, conjugation to a highly negatively charged dye can dramatically alter an antibody’s isoelectric point (iP), which is essential for maintaining specific antibody-antigen interaction. With this insight, Biotium scientists devised a revolutionary new approach to near-IR dye design that results in superior physical properties of the dyes without introducing an excessive amount of negative charge.

Biotium’s near-IR CF™ dyes are based on the core structure of either cyanine dyes or rhodamine dyes. Those core structures are modified such that the intramolecular mobility of the dyes is restricted, which leads to higher quantum yield and better water solubility without adding excessive charge. As a result, near-IR CF™ dyes are much brighter and more photostable than any other near-IR dyes. Most importantly, antibodies labeled with near-IR CF dyes™ give far better signal-to-noise ratio in immunostaining compared with antibody conjugates prepared with other commercial near-IR dyes.

Where can I find more information about US Biological?

Visit the manufacturer page at www.stratech.co.uk/usbio, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.