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Tuesday, February 24, 2015

Stratech Scientific UK Seminar Series

In the next few weeks Stratech Scientific will be holding a number of talks around the UK, to find out if there is one near you and the topics visit our Facebook events page our check out our meetings page.

All Events are by Invite Only

Please contact Gareth Bolitho (Sales Manager at Stratech Scientific) at gareth@stratech.co.uk to organise a pass and for any questions regarding the events or the technologies presented.

Application Examples of SynVivo Technology in Action

SynVivo products are a physiological, cell-based microchip platform that provides a morphologically and biologically realistic microenvironment allowing real-time study of cellular behavior, drug delivery and drug discovery.

SynVivo’s patented technology for recreating microvasculature scale, shape, fluidics, and cellular constructs provides the most realistic in vitro testing platform for drug discovery, delivery, efficacy, and toxicity assays.

The examples below are specific applications of SynVivo’s technology to set examples where this system can bridge the gap to in vivo.


 

Migration Assays

Imagine an assay which is repeatable, simple to use, provides real-time live cell imaging and gives data which matches in vivo observations. SynVivo has overcome the limitations of Transwell® assays to provide an entirely new system for studying migration in a realistic and dynamic environment.



 

Nanopolymer Delivery for Gene Therapy

A nanopolymer used for drug delivery was complexed with GFP DNA and perfused on tumor cell (HeLa) cultured networks. GFP transfection, efficiencies and distribution was quantified at 24 hours post injection.



 

Targeted Drug Delivery

A confluent layer of endothelial cells were activated with cytokines. Anti-ICAM-1 coated particles were used for targeting (red dots) and IgG particles served as controls (green dots). Data was obtained at 4 and 24 hours post treatment.



 

Drug Particle Adhesion

P-selectin, an inflammatory molecule was coated on the walls of SynVivo. Particles coated with the antibody to P-selectin were injected into SynVivo and their differential binding pattern was observed.



 

Drug Perfusion

SynVivo were injected with trypan blue and the perfusion of the dye was observed in real-time.




Where can I find more information about SynVivo?

Visit the manufacturer page at www.stratech.co.uk/synvivo, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.


Thursday, February 19, 2015

Product Focus: Magnetic Beads & Agarose from Solulink

Highest biotin-binding streptavidin magnetic beads and agarose on the market
Advantages
  • High binding capacity – Higher biotin binding capacity at >20% lower price
  • Crosslinked agarose – Solulink’s proprietary linker enables higher binding capacity, lower background, and less leaching
  • Multiple sizes – Available in 2 mL, 5 mL, 10 mL, and bulk quantities

Applications
  • Immobilization of biotinylated biomolecules or drugs
  • Immunoprecipitation, ChIP, cell capture, and others
  • Cell-surface labeling with biotinylation reagents, followed by precipitation with streptavidin
  • Recovery of biotinylated DNA for dideoxy sequencing

Over the past 10 years, Solulink’s streptavidin-coupled NanoLink™ and MagnaLink™ Beads have been widely used in types of organizations and are regularly cited in papers for diverse manual and automated applications. Watch the video to learn more:

Solulink offers streptavidin magnetic beads and agarose with the highest biotin-binding capacity on the market – beads with as much as 12X higher binding capacity and agarose with 20% lower price. Higher binding translates into reduced bead mass or agarose required to immobilize a biotinylated sample and lower background noise from nonspecific binding, resulting in better signals and lower net costs.

Molecule

NanoLink (0.8 um) Binding Capacity

MagnaLink (2.8 um) Binding Capacity

Free biotin
>14 nmol/mg
>12 nmol/mg
Biotinylated oligo (23-mer)
>2.5 nmol/mg
>0.8 nmol/mg
Biotinylated IgG (3 biotins per IgG)
>250 ug/mg
>112.6 ug/mg

The secret is in the crosslinking

The key to high biotin binding is in the unique covalently crosslinked streptavidin, based on Solulink’s proprietary linking technology. The high surface area, when combined with our efficient linking chemistry, produces a consistent, ultra-high, biotin-binding bead.




 

Streptavidin Agarose Ultra Performance

 

High biotin-binding streptavidin agarose

Solulink’s Streptavidin Agarose Ultra Performance™ provides high biotin binding at a low price. We used Solulink’s proprietary linking technology coupled with a 6% highly crosslinked agarose to boost the biotin binding capacity of the high specific activity streptavidin. This ideal combination provides a biotin binding capacity of >330 nmol/mL of resin—one of the highest loading-capacity products currently available. Use Solulink’s Streptavidin Agarose Ultra Performance™ for improved recovery of any biotinylated biomolecule and you can lower nonspecific binding, lower costs, and get better results.

Application Note: How to Capture at Least 2x More Antigen in an Immunoprecipitation


 

Comparative Binding Data


 

Products

Cat. No. Product Name Size
M-1002-010 NanoLink™ Streptavidin Magnetic Beads 0.8 μm 1 mL at 10 mg/mL
M-1002-020 NanoLink™ Streptavidin Magnetic Beads 0.8 μm 2 mL at 10 mg/mL
M-1002-050 NanoLink™ Streptavidin Magnetic Beads 0.8 μm 5 mL at 10 mg/mL
M-1002-100 NanoLink™ Streptavidin Magnetic Beads 0.8 μm 10 mL at 10 mg/mL
M-1002-1000 NanoLink™ Streptavidin Magnetic Beads 0.8 μm 100 mL at 10 mg/mL: Please Inquire for Pricing
M-1001-010 NanoLink™ 4FB Magnetic Beads 0.8 μm 1 mL at 10 mg/mL
M-1000-001 NanoLink™ Amino Magnetic Beads 0.8 μm 1mL at 10 mg/mL
M-1003-010 MagnaLink™ Streptavidin Magnetic Beads 2.8 µm 1 mL at 10 mg/mL
M-1003-020 MagnaLink™ Streptavidin Magnetic Beads 2.8 µm 2 mL at 10 mg/mL
M-1003-050 MagnaLink™ Streptavidin Magnetic Beads 2.8 µm 5 mL at 10 mg/mL
M-1003-100 MagnaLink™ Streptavidin Magnetic Beads 2.8 µm 10 mL at 10 mg/mL
M-1003-1000 MagnaLink™ Streptavidin Magnetic Beads 2.8 µm 100 mL at 10 mg/mL: Please Inquire for Pricing
M-1004-010 MagnaLink™ 4FB Magnetic Beads 2.8 μm 1 mL at 10 mg/mL
M-1005-010 MagnaLink™ Amino Magnetic Beads 2.8 µm 1mL at 10 mg/mL
N-1000-002 Streptavidin Agarose Ultra Performance 2 mL
N-1000-005 Streptavidin Agarose Ultra Performance 5 mL
N-1000-010 Streptavidin Agarose Ultra Performance 10 mL


Select Citations from Top Researchers

NanoLink™ and MagnaLink™ Streptavidin Magnetic Beads
  • Das A, Goldstein JL, Anderson DD, et al. Use of mutant 125I-perfringolysin O to probe transport and organization of cholesterol in membranes of animal cells. Proc. Natl. Acad. Sci. U.S.A. 2013 Jun 25;110(26):10580–10585. Link
  • Steele S, Brunton J, Ziehr B, et al. Francisella tularensis Harvests Nutrients Derived via ATG5-Independent Autophagy to Support Intracellular Growth. PLoS Pathog. 2013 Aug;9(8):e1003562. Link
  • Flanigon J, Kamali-Moghaddam M, Burbulis I, et al. Multiplex protein detection with DNA readout via mass spectrometry. N Biotechnol. 2013 Jan 25;30(2):153–158. Link
  • Tanabe C, Maeda T, Zou K, et al. The Ubiquitin Ligase Synoviolin Up-regulates Amyloid β Production by Targeting a Negative Regulator of γ-Secretase, Rer1, for Degradation. J. Biol. Chem. 2012 Dec 28;287(53):44203–44211. Link
  • Fowler DM, Araya CL, Fleishman SJ, Kellogg EH, Stephany JJ, Baker D, Fields S. High-resolution mapping of protein sequence-function relationships. Nature Methods 2010;7(9):741-6. Link
  • Kuzmin A, Poloukhtine A, Wolfert MA, Popik VV. Surface functionalization using catalyst-free azide-alkyne cycloaddition. Bioconjug Chem. 2010;21(11):2076-85. Link
  • Fan WQ, Morinaga H, Kim JJ, Bae E, Spann NJ, Heinz S, Glass CK, Olefsky JM. FoxO1 regulates Tlr4 inflammatory pathway signalling in macrophages. The EMBO Journal 2010;29(24):4223-36. Link
  • Branchini BR, Ablamsky DM, Rosenberg JC. Chemically modified firefly luciferase is an efficient source of near-infrared light. Bioconjug Chem. 2010;21(11):2023-30. Link
  • Jensen RB, Carreira A, Kowalczykowski SC. Purified human BRCA2 stimulates RAD51-mediated recombination. Nature 2010;467(7316):678-683. Link
  • Fowler DM, Araya CL, Fleishman SJ, Kellogg EH, Stephany JJ, Baker D, Fields S. High-resolution mapping of protein sequence-function relationships. Nature Methods 2010;7(9):741-6. Link
  • Kuzmin A, Poloukhtine A, Wolfert MA, Popik VV. Surface functionalization using catalyst-free azide-alkyne cycloaddition. Bioconjug Chem. 2010;21(11):2076-85. Link
  • Fan WQ, Morinaga H, Kim JJ, Bae E, Spann NJ, Heinz S, Glass CK, Olefsky JM. FoxO1 regulates Tlr4 inflammatory pathway signalling in macrophages. The EMBO Journal 2010;29(24):4223-36. Link
  • Branchini BR, Ablamsky DM, Rosenberg JC. Chemically modified firefly luciferase is an efficient source of near-infrared light. Bioconjug Chem. 2010;21(11):2023-30. Link
  • Jensen RB, Carreira A, Kowalczykowski SC. Purified human BRCA2 stimulates RAD51-mediated recombination. Nature 2010;467(7316):678-683. Link
  •  


Where can I find more information about Solulink?

Visit the manufacturer page at www.stratech.co.uk/solulink, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.