Wednesday, July 1, 2015

Supplier Focus: Creative Bioarray – Stem Cell Offering

Creative Bioarray offers over 1,000 stem cell products including adult stem cells, tumour stem cells, embryonic stem cells, iPS cells and other stem cells.

Adult Stem Cells are there to repair any damage that has been done to tissue and rebuild it back to its original state. We have many samples from different organs and systems of mice and rats.

Tumour Stem Cells samples are extremely valuable to those who need to compare them to normal stem cells of a certain origin and study how they give rise to cancerous cells.

Embryonic Stem Cells are available for both human and mouse embryos.

iPS Cells are cells that are derived from adult cells and are extremely useful in modeling diseases and drug development.


 

Adult Stem Cells

Adult stem cells are undifferentiated cells, found throughout the body after development that multiply by cell division to replenish dying cells and regenerate damaged tissues. Also known as somatic stem cells, they can be found in juvenile as well as adult animals and human bodies. Creative Bioarray provides adult stem cells from various tissue/organs of human, mouse and rat.
  • Human Adult Stem Cells
  • Mouse Adult Stem Cells
  • Rat Adult Stem Cells
  • Dog Adult Stem Cells
  • Rabbit Adult Stem Cells

Tumour Stem Cells

Tumour stem cells are cancer cells (found within tumors or hematological cancers) that possess characteristics associated with normal stem cells, specifically the ability to give rise to all cell types found in a particular cancer sample. Creative Bioarray offers tumor stem cells representing more than 20 types of carcinoma.

Embryonic Stem Cells

Embryonic stem cells (ES cells) are pluripotent stem cells derived from the inner cell mass of a blastocyst, an early-stage preimplantation embryo. Creative Bioarray provides embryonic stem cells from both human and mouse.
  • Human Embryonic Stem Cells
  • Mouse Embryonic Stem Cells
  • Rat Embryonic Stem Cells

iPSC

Induced pluripotent stem cells (also known as iPS cells or iPSC) are a type of pluripotent stem cell that can be generated directly from adult cells. We offer human iPSC lines derived from various primary cells by different inducing methods.

Other Stem Cells

Creative Bioarray also provides cells differentiated from stem cells.



Where can I find more information about Creative Bioarray?

Visit the manufacturer page at www.stratech.co.uk/creative-bioarray, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.

Supplier Focus: Biotool – cDNA Synthesis SuperMix

Biotool offers a range of optimized cDNA synthesis systems (SuperMix) to generate cDNA for a variety of research interests.

All-in-One cDNA Synthesis SuperMix


BioScript All-in-One cDNA Synthesis SuperMix provides first-strand cDNA synthesis, optimized with the high-temperature capability of Reverse Transcriptase and an optimized SuperMix formula. In one tube, the pre-blended 5× qRT SuperMix contains all the necessary components for reverse transcription of YOUR RNA template. The resulting cDNA product is suitable for use in real-time quantitative RT-PCR (qRT-PCR).


  • Reproducibility: Modified Oligo dT: Random primers ratio facilitates qPCR repeatability.
  • High compatibility: cDNA product is suitable for application with SYBR Green or Probe qPCR.
 


 

SuperFast cDNA Synthesis SuperMix


SuperFast cDNA Synthesis SuperMix is designed for cDNA synthesis by genetic modification. Reverse transcription can be completed within 5 minutes, due to high affinity with RNA templates and a fast extension rate. The cDNA is efficiently synthesized by incubating the SuperFast RT/RI Enzyme Mix with the optimized 2 × TS SuperFast Reaction Mix. The cDNA products are suitable for downstream PCR and qPCR applications.


  • Fast synthesis: Only 5 minutes needed for reverse transcription.
  • Higher yield: Save RNA from degradation due to the fastest reverse transcription.
  • Size of cDNA: Up to 12 kb in product length, more full-length cDNA is obtained.

 


 

SuperZyme III cDNA Synthesis SuperMix


SuperZyme III cDNA Synthesis SuperMix provides all the necessary components for cDNA synthesis from total RNA or mRNA. The cDNA is efficiently synthesized by employing SuperZyme III RT/RI Enzyme Mix and an optimized 2×TS III Reaction Mix. RNase H-Deficiency reduces RNA template degradation during the 1st-strand cDNA synthesis. Product lengths of cDNA can be up to 15 kb. The cDNA products are suitable for downstream PCR, qPCR and RNA-sequencing applications.


 



ThermoStable cDNA Synthesis SuperMix


ThermoStable cDNA Synthesis SuperMix (+gDNA Remover) is engineered to have high thermal stability (up to 65°C) for efficient transcription of RNA regions with high secondary structure. It is stable over a broad range of reaction temperatures (42°C-65°C) and can achieve simultaneous genomic DNA (gDNA) removal and cDNA synthesis in one tube. This product generates the highest yields of cDNA, and is able to synthesize very long RNA transcripts up to 20 kb. The cDNA products are suitable for downstream PCR, qPCR and RNA-Sequencing applications.


High thermostability: ThermoStable Reverse Tramscriptase is active up to 65°C.
Double advantage: Simultaneous gDNA removal and cDNA synthesis.
Higher yield: Synthesize very long RNA transcripts up to 20 kb.

 



Where can I find more information about Biotool?

Visit the manufacturer page at www.stratech.co.uk/biotool, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.

Supplier Focus: Biotool – Hot Start High-Fidelity DNA Polymerase

Universe Hot Start High-Fidelity DNA polymerase

User GuideMSDS

Universe High-Fidelity Hot Start DNA polymerase is a superior enzyme in this category for robust PCR with extreme fidelity, featuring 53x error rate lower than Taq polymerase and extension rate as fast as 1 sec / kb*. Universe High-Fidelity Hot Start DNA polymerase possesses 5´→ 3´polymerase activity, 3´ → 5´exonuclease activity and will generate blunt-ended products. The polymerase is also capable of amplifying long fragments such as 20kb genomic DNA and 40kb λDNA. Our suggested applications include cloning, sequencing, genomic DNA amplification and high throughput PCR, etc.

Advantages

  • Robust: Maximal success with minimal optimization
  • Extreme Fidelity: 53x error rate lower than Taq
  • High Yield: Increased product yield using minimal enzyme
  • Versatile: Used for routine PCR, or with long, difficult templates

 

Materials Provided

Component B21101 100U (100 runs) B21102 250U (250 runs) B21103 500U (500 runs)
Universe High-Fidelity Hot Start DNA polymerase (1 U /μl) 100 μL 250 μL 500 μL
2 x Universe buffer 1.25 mL x 2 1.25 mL x 5 1.25 mL x 10
dNTP Mix (10mM each) 100 μL 250 μL 500 μL
10 x Loading buffer 1.25 mL 1.25 mL x 3 1.25 mL x 5

 

Universe Hot Start High-Fidelity 2x PCR Master Mix

Universe Hot Start High-Fidelity 2x PCR Master Mix

Biotool Universe Hot Start High-Fidelity 2x PCR Master Mix is a convenient premix version of Universe High-Fidelity Hot Start DNA Polymerase, which is a superior enzyme in this category for robust PCR with extreme fidelity, featuring 53x error rate lower than Taq polymerase and extension rate as fast as 1 sec / kb*. Universe High-Fidelity Hot Start DNA Polymerase possesses 5´→ 3´polymerase activity, 3´ → 5´exonuclease activity and will generate blunt-ended products. The polymerase is also capable of amplifying long fragments such as 20kb genomic DNA and 40kb λDNA. Our suggested applications include cloning, sequencing, genomic DNA amplification and high throughput PCR, etc.

* The extension rate varies with individual application.

Advantages

  • Robust: Maximal success with minimal optimization
  • Extreme Fidelity: 53x error rate lower than Taq
  • High Yield: Increased product yield using minimal enzyme
  • Versatile: Used for routine PCR, or with long, difficult templates
  • Convenient: Premix allows rapid reaction set-up

 

Materials Provided


Component B22101 (100 rxns) B22102 (250 rxns) B22103 (500 rxns)
Universe Hot Start High-Fidelity 2x PCR Master Mix 1.25mL x 2 1.25mL x 5 1.25mL x 10

 

Amplification Chart


Fig1: Amplification of genomic DNA templates of various lengths(0.6kB, 1.0kb, 2.6kb, 3.0kb, 4.0kb, 5.1kb, 6.2kb, 7.1kb, 8.5kb, 10.6kb, 17.8kb, 20.3kb, 21.4kb) using Universe High-Fidelity Hot Start DNA polymerase at extension rate of 15 seconds/kb.

Fig2: Amplification of λDNA and human genome DNA templates at various extension rate of 1sec/kb, 2 sec/kb, 5 sec/kb, 10 sec/kb, 15 sec/kb, 30 sec/kb, 60 sec/kb, respectively.



Where can I find more information about Biotool?

Visit the manufacturer page at www.stratech.co.uk/biotool, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.