|Figure 1. DNA fragments ranging from 1 kb to 6 kb amplified from λ DNA.
Properties: Activator: Mg2+
Purity: Free from detectable non-specific nucleases.
Storage Buffer: 20mM Tris-HCl, pH 8.5, 1mM DTT, 0.1mM EDTA, 100mM KCI, 50% glycerol, stabilizers.
Unit Definition: One unit incorporates 10 nmol of total nucleotides into acid-insoluble material in 30 min at
74°C in a total volume of 50 μl.
Concentration: 5 units/μl
Assay Conditions: The reaction mixture (50 μl) contains 25mM TAPS, pH 9.3 (at 25°C), 50mM KCl, 2mM MgCl2, 1mM 2-mercaptoethanol, 200μM dNTPs, 250 μg/ml activated salmon sperm DNA, and Taq DNA Polymerase. After incubation at 74°C for 10 min, acid insoluble material is determined.
Functional Test: Tested in a standard PCR.
Functionally Tested 10X PCR Reaction Buffer (included, PN 71165): 100mM Tris-HCl (pH 8.6), 500mM KCl, 15mM MgCl2
Functionally Tested MgCl2 (included, PN 71167): 25mM solution
ProductsFunctionally tested and has no detectable contaminating endonucleases or exonucleases
Where can I find more information about Affymetrix USB?
Visit the manufacturer page at www.stratech.co.uk/affymetrix, email email@example.com or call +44 (0) 1638 782600.