Thursday, February 21, 2013

Cell Explorer™ Live Cell Labeling Kit (Green Fluorescence)

AAT Bioquest Cell Explorer™ fluorescence imaging kits are a set of tools for labeling cells for fluorescence microscopic investigations of cellular functions. The effective labeling of cells provides a powerful method for studying cellular events in a spatial and temporal context. This particular kit is designed to uniformly label live cells in green fluorescence. The kit uses non-fluorescent Calcein Green™ that becomes strongly fluorescent upon entering into live cells. Calcein Green™ is a hydrophobic compound that easily permeates intact live cells. The hydrolysis of the non-fluorescent Calcein Green™ by intracellular esterases generates the strongly fluorescent hydrophilic Calcein Green™ fluorophore that is well-retained in the cell cytoplasm. Cells grown in black-walled plates can be stained and quantified in less than two hours. It can be readily adapted for high-throughput assays in a wide variety of fluorescence platforms such as microplate assays, immunocytochemistry and flow cytometry.  It is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. The kit provides all the essential components with an optimized cell-labeling protocol. It is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells.

Detailed Information:   Protocol      References      MSDS      Spectrum
Product Code 22607 Storage F/D/L (see storage codes)
Unit Size 1 kit Em (nm) 515
Ex (nm) 495




Where can I find more information about AAT Bioquest?

Visit the manufacturer page at www.stratech.co.uk/aat, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.
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