Wednesday, March 6, 2013

Antibody-Oligonucleotide All-in-One Conjugation Kit

No chromatography required!
The first easy-to-use kit to conjugate antibodies with oligos

The Antibody-Oligonucleotide All-in-One Conjugation Kit offers an innovative TurboLink™ catalyzed conjugation based on Solulink’s proprietary linking technology to prepare antibody-oligonucleotide conjugates without chromatography. This novel kit includes buffers, spin columns, and a calculator to determine MSR. No column chromatography is required. With the Antibody-Oligonucleotide All-in-One Conjugation Kit, you can generate high-purity conjugates virtually free of residual antibody or oligonucleotide (>95% conjugate).

 Download White Paper: Antibody-Oligonucleotide Conjugate Preparation 

How it works
Solulink’s proprietary linking technology is based on the use of two complementary heterobifunctional linkers: 
  1. Amine-modified, 20 to 60-mer oligonucleotide is modified using an excess of the Sulfo-S-4FB linker. This reactive NHS-ester incorporates a 4FB (aromatic aldehyde functional group, formylbenzamide) at the desired terminus of the oligonucleotide.
  2. Polyclonal or monoclonal antibody (100 μg) is modified using the S-HyNic linker. This NHS-ester reacts with lysine residues, incorporating HyNic functional groups (hydrazino-nicotinamide) onto the antibody.
  3. The two modified biomolecules are mixed together in the presence of the TurboLink™ catalyst, aniline, leading to rapid and efficient conversion of the antibody to conjugate through formation of stable bis-arylhydrazone bonds, followed by magnetic-affinity, solid phase purification.
  4. The antibody-oligonucleotide conjugate is ready for use in the most demanding and sensitive applications.

Advantages
  • Simple and Easy to Use – Requires only micropipette, microcentrifuge, and UV spectrophotometer
  • Automated Calculations – Calculator with fully integrated input/output provided
  • High Yield – 30–50% yield based on starting antibody
  • High Purity –  >95% purity without chromatographic purification
  • High Stability – Conjugates are stable for >1 year

Where can I find more information about Solulink?

Visit the manufacturer page at www.stratech.co.uk/solulink, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.
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