Tuesday, March 12, 2013

Chemical Environment - JBScreen Solubility HTS


01.03.2013

Chemical Environment - JBScreen Solubility HTS

Jen Bioscience supply Meindert Lamers JBScreen Solubility HTS tests, designed to quickly find suitable buffer components to purify and store protein in.
 
Description
JBScreen Solubility HTS tests for buffer, pH, salt and glycerol at the same time: For all proteins investigated, suitable conditions were found in a single assay. Protein and buffer conditions are dispensed in a ratio of 1:3, thereby minimizing the effect of any buffer components in which the protein is initially stored. Standard crystallization robots set up the assay in 5 minutes and very small amounts of protein are required (10 ┬Ál @ 5-10 mg/ml). The results are visible after one hour at high protein concentration or 12 hours at low protein concentration:
 
Examples of a drop without protein precipitation (left) and a drop with protein precipitation (right)
 
 

Product Cat. No. Amount Downloads
JBScreen Solubility HTS CO-311
95 solutions
(1.7 ml each)
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