Thursday, April 18, 2013

Human Mesenchymal Stem Cells-Green Fluorescent Nanoparticle-labeled

These cells are generated by chemical transfection of CdSe/ZnS nanoparticles, 100 nm in diameter and show a strong fluorescent signal (Excitation maximum= 515 nm; Emission maximum= 520-550 nm) detectable by standard FITC filters.

Technical Information & Data.

They retain GFNP labeling through 3+ passages and can be differentiation into chondrogenic, adipogenic or osteogenic lineages. Excellent for regeneration, toxicity and related studies.

Picture
FITC analysis of cells during fourth passage following transfection. Image obtaining using FITC filter on Olympus CKX41 microscope at 200 X equipped with a Retiga 2000 digital camera and Q-Capture software program. Scale bar is 25 micrometers. Fluorescent nanoparticles are less prevalent within the cytoplasm than earlier passages.

Optimizing your hMSC Cultures:
1)     Grow cells in 1 to 5% O2, 5% CO2, with the balance of the gas content as N2..
2)      Use of our MSCGroTM media is highly recommended.
3)     Subtrate- We recommend Greiner Bio-one TC-treated cell culture flasks or equivalent.
Picture[2]
Osteogenic differentiation of GF nanoparticle-labeled MSCs. Image obtaining using phase contrast Olympus CKX41 microscope at 100 X equipped with a Retiga 2000 digital camera and Q-Capture Pro software program. Scale bar is 25 micrometers. Note the presence of mineral deposits indicating differentiation into osteoblasts. Chondrogenic and adipogenic differentiation was also apparent.

Catalog Number Size
SC00A4NP 500,000
Type: Stem Cells
Applications: MSC Cell Assays
Species Reactivity: Human
Format: Frozen

Where can I find more information about Neuromics?

Visit the manufacturer page at www.stratech.co.uk/neuromics, email info@stratech.co.uk or call  +44 (0) 1638 782600.
Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe.
Please contact us to find out which product ranges we can supply in your country.
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