Accumulation of beta-amyloid as plaques between neurons in the brain is a primary pathological feature of Alzheimer’s disease. Amyloid is a general term for protein fragments produced normally by the body. Beta-amyloid is a protein fragment snipped from an amyloid precursor protein (APP). In a healthy brain these protein fragments are broken down and eliminated, but in Alzheimer’s disease the fragments accumulate to form hard, insoluble plaques. These plaques have been correlated with neurotoxicity and the cognitive decline associated with the disease.
Reliable, consistent sources of ‘artificial‘ beta-amyloid are essential to scientists researching the role of this peptide in its various forms, in relation to Alzheimer’s disease. The most common artificial beta-amyloid is chemically synthesised, but does have disadvantages to the researcher.
When a synthetic beta-amyloid is made, the final full length peptide is in a crude prep mixture. Beta-amyloid is a highly hydrophobic peptide and binds non-specifically to impurities like heavy metals, which are not detectable on HPLC or Mass Spec, but could alter the biological properties of the peptide. In every synthetic preparation this will vary, leading to variability in the biological properties from lot to lot.
rPeptide’s recombinant beta-amyloid peptides are prepared as a very soluble (proprietary) fusion, to significantly reduce the non-specific binding in an impure prep. This soluble fusion is purified to >90% purity and then cleaved to give the final beta-amyloid peptides, which are further purified to >97% purity. As a result, rPeptide’s recombinant beta-amyloids offer greater batch to batch reproducibility.
- Batch to Batch Consistency: of Physical, Chemical and Biological Properties. This is THE biggest problem with synthetic beta-amyloid peptides and is not found with recombinant beta-amyloids.
- Purity: Consistently >97%
- Oxidation: No oxidation of the 35Met. A mutant 35Met-Valine, is available to study the effect of oxidation of methionine.
- Chemical Modifications: None (like racemerisation).
- Uniformly full length peptides: No n-1, n-2 subspecies in each lot.
- Uniformly Labelled Peptides: Can economically and uniformly label with 15N, 13C and 15N+13C
- Net Peptide Content: Recombinant Beta-amyloid is sold as “net” peptide content rather than ‘total’ content. Peptides sold as ‘total’ content often include 20%-40% salt.
- Available in a range of salts allowing you to choose the optimal format for your research (HCl, NaOH, HFIP, TFA)
Where can I find more information about rPeptide?
Visit the manufacturer page at www.stratech.co.uk/rpeptide, email firstname.lastname@example.org or call +44 (0) 1638 782600.
Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.