Monday, March 3, 2014

Product Focus: ExoQuick the Exosome Precipitation Reagent

Exosomes are 60 – 150 nm membrane vesicles secreted by most cell types in vivo and in vitro. Exosomes are found in blood, urine, amniotic fluid, malignant ascite fluids and contain distinct subsets of microRNAs depending upon the tumor from which they are secreted. SBI’s ExoQuick exosome precipitation reagent makes microRNA and protein biomarker discoveries simple, reliable and quantitative. Enrich for circulating exosomal microRNAs with ExoQuick and accurately profile them using SBI’s SeraMir™ qPCR arrays.

 

Benefits

  • No time-consuming ultra centrifugation
  • Less expensive than costly Antibodies and beads
  • No complicated syringes required
  • Compatible with biofluid from any species
  • More effective than any other method
  • Isolate intact exosomes for functional studies
  • Use as little as 100 µl of serum
  • Start with 5 or 10 ml Media or Urine and use ExoQuick-TC

 

How to use ExoQuick

 

Amounts of ExoQuick to add to various biofluids

 

ExoQuick exosomes can be transfered between cells

SBI created a stable 293TN cell line overexpressing the Cyto-Tracer™ pCT-CD63-GFP fusion protein (catalogue CYTO120-PA-1). The media from the cells was collected 48 h after plating and the exosomes from the media were precipitated using ExoQuick-TC. The exosome pellet recovered was resuspended in 30ul PBS and 10ul was added to newly plated HT1080 cells. HT1080 cells were visualized 72 h after the addition of the CD63-GFP labeled exosomes and then re-plated. Following another 24h, the cells were again visualized for GFP fluorescence and imaged. The exosomes appear to dock with the cells within 72 h and some are found to be internalized after 96 h.



Where can I find more information about Sytsems Biosciences?
Visit the manufacturer page at www.stratech.co.uk/sbi, email info@stratech.co.uk or call +44 (0) 1638 782600.
Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.
Post a Comment