Thursday, April 24, 2014

Product Focus: Thermophilic Polymerases for Maximum Flexibility

Jena Bioscience offers a broad range of optimized thermophilic polymerases. Make your selection from Taq polymerases for routine PCR applications, hot start polymerases for high specific amplifications or proofreading enzyme blends for high fidelity and long range PCR. All enzymes ensure reliable, high performance results and guarantee maximum success for their particular application.

Standard PCR – Product Selection Guide


 

Taq Pol – Thermostable DNA polymerase

 


 

Taq Pol / high yieldThermostable DNA polymerase

Taq Pol / high yield is recommended for use in routine PCR reactions. The buffer system is optimized for high efficiency and gives superior amplification results in a broad range of reaction conditions with most primer-template pairs. The buffer system facilitates the incorporation of labeled or modified nucleotides into DNA. Note that the ammonium based buffer contains detergents and may interfere with automated pipetting systems.
The enzyme replicates DNA at 72°C. It catalyzes the polymerization of nucleotides into duplex DNA in 5′→3′ direction in the presence of magnesium. It also possesses a 5′→3′ polymerization-dependent exo-nuclease replacement activity but lacks a 3′→5′ exonuclease activity.
Taq Pol – red / high yield buffer contains an inert red dye for easier handling and to facilitate the preparation of the master mix.


 

Hot Start PolHeat-activatable DNA polymerase for high specificity

  • Hot Start Pol provides improved specificity and sensitivity when amplifying low-copy-number targets in complex backgrounds or when prolonged room-temperature set up is required. The polymerase activity is blocked at ambient temperature and switched on automatically at the onset of the initial denaturation. The thermal activation prevents the extension of nonspecifically annealed primers and primer-dimer formation at low temperatures during PCR setup.
    The enzyme catalyzes the polymerization of nucleotides into duplex DNA in 5′→3′ direction in the presence of magnesium. It also possesses a 5′→3′ polymerization-dependent exonuclease replacement activity but lacks a 3′→5′ exonuclease activity. 
  • Hot Start Pol requires no prolonged activation step. The polymerase inhibiting ligand is quickly released at the increased temperature of thermal cycling. 
  • Hot Start Pol – red contains an inert red dye for easier handling and to facilitate the preparation of the master mix.


 

Rapid Pol - Thermostable DNA polymerase for rapid PCR

Rapid Pol is based on a specifically processed enzyme engineered for higher processivity and speed, offering significantly faster extension rates than wild-type Taq polymerase.
The rapid polymerase is designed for Fast PCR, in which total reaction times are 20-70% shorter compared with conventional PCR assays. This can be achieved without sacrificing reaction performance or the requirement for specialized PCR consumables or thermocyclers.


 

High Fidelity PolDNA polymerase for high accuracy

High Fidelity Pol is based on a blend of Taq DNA polymerase and a proofreading enzyme specially designed for highly accurate and efficient amplification. It shows excellent results with extremely long (up to 30 kb), GC-rich or other difficult templates.
The enzyme blend includes a highly processive 5′→3′ DNA polymerase and possesses a 5′→3′ polymerization-dependent exonuclease replacement activity. Its inherent 3′→5′ exonuclease proofreading activity results in a greatly increased fidelity of DNA synthesis compared to Taq polymerase.
The enzyme is highly purified and free of bacterial DNA.


 

High Fidelity Hot Start PolHeat-activatable DNA polymerase for high accuracy and specificity

High Fidelity Hot Start Pol is based on a blend of Taq DNA polymerase and a proofreading enzyme specially designed for highly accurate and efficient amplification. The additional hot-start function provides improved specificity and sensitivity when amplifying low-copy-number targets in complex backgrounds or when prolonged room-temperature set up is required. The polymerase activity is blocked at ambient temperature and switched on automatically at the onset of the initial denaturation. The thermal activation prevents the extension of nonspecifically annealed primers and primer-dimer formation at low temperatures during PCR setup.
The enzyme shows excellent results with extremely long (up to 30 kb), GC-rich or other difficult templates.
Activation step
High Fidelity Hot Start Pol requires no prolonged heating or denaturing step. The polymerase inhibiting antibodies are released at the increased temperature of the initial denaturation.


 

Pfu-X PolymeraseProofreading DNA polymerase for highest accuracy

Pfu-X Polymerase is the ideal choice for applications where the efficient amplification of DNA with highest fidelity is required.
The enzyme is a genetically engineered Pfu DNA polymerase, but showing a 2-fold higher accuracy and an increased processivity, resulting in shorter elongation times.
The enzyme catalyzes the polymerization of nucleotides into duplex DNA in 5′→3′ direction but does not possess a 5′→3′ exonuclease replacement activity. Its inherent 3′→5′ exonuclease proofreading activity results in a greatly increased fidelity of DNA synthesis compared to Taq polymerase. Pfu-X Polymerase-generated PCR fragments are blunt-ended.
The enzyme is highly purified and free of bacterial DNA.


 

Sequencing Pol - Taq Pol mutant for incorporation of ddNTPs

Sequencing Pol is a Taq polymerase mutant showing an enhanced efficiency for incorporation of ddNTPs. Its capability of incorporating ddNTPs and dNTPs at equal rates makes it the ideal choice for DNA cycle sequencing.
The enzyme replicates DNA at 74°C. It catalyzes the polymerization of nucleotides into duplex DNA in 5′→3′ direction in the presence of magnesium and has a 5′→3′ polymerisation-dependent exonuclease replacement activity only.



Where can I find more information about Jena Bioscience?
Visit the manufacturer page at www.stratech.co.uk/jena_bioscience, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.


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