Friday, April 4, 2014

Product Focus: VeriQuest Probe qPCR Master Mix

VeriQuest Probe qPCR Master Mix is supplied as a 2X pre-mixed formulation containing both ROX and UDG in an optimized buffer for quality results in real-time quantitative PCR assays. The proprietary reaction buffer with optimum MgCl2 concentration is specifically designed for robust probe hybridization and efficient cleavage of TaqMan probes. Simply add DNA template, primers, probe(s), and water and the reactions are ready for cycling. The master mix contains a chemically-modified Taq DNA Polymerase, MgCl2, ultrapure nucleotides with an optimized dUTP:dTTP ratio, Uracil-DNA Glycosylase (UDG or UNG), and ROX Passive Reference Dye in a proprietary reaction buffer.

Features:
  • One-tube master mix with ROX Reference Dye included
  • Similar protocols, same probes, and same primers for standard mode amplification
  • Consistency over a broad dynamic range: 9 orders of magnitude linear detection range with a correlation coefficient = 0.999
  • Exceptional performance with challenging GC-rich regions
  • Detect 2 copies of target from genomic DNA and differentiate less than a 2-fold differences
  • Contains dUTP and Uracil-DNA Glycosylase (UDG or UNG) for optional carryover contamination prevention.
  • Hot start PCR for room temperature reaction setup
  • Stable at room temperature for 72 hours in a preassembled reaction
  • Validated for use with a variety of 5’ reporter fluorophores and 3’ quenchers, as well as TaqMan MGB containing probes



Simplify the process with a single tube master mix containing UDG and ROX passive reference dye

Catalogue No. Description Unit Size
75650_40_RXN-AFM
VeriQuest Probe qPCR Master Mix (2X)
1 ml for 40 reactions (50 µl)
75650 200 RXN-AFM
VeriQuest Probe qPCR Master Mix (2X)
5 ml for 200 reactions (50 µl)
75650 400 RXN-AFM
VeriQuest Probe qPCR Master Mix (2X)
2 x 5 ml for 400 reactions (50 µl)
75650 1000 RXN-AFM
VeriQuest Probe qPCR Master Mix (2X)
5 x 5 ml for 1,000 reactions (50 µl)
75650 2000 RXN-AFM
VeriQuest Probe qPCR Master Mix (2X)
10 x 5 ml for 2,000 reactions (50 µl)

Literature




Where can I find more information about Affymetrix USB?

Visit the manufacturer page at www.stratech.co.uk/affymetrix, email info@stratech.co.uk or call +44 (0) 1638 782600.
 
Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.
Post a Comment