Tuesday, September 30, 2014

Cost-effective and easy to handle biotin removal

Biotin is often present in cell culture media. Its nearly irreversible binding to Strep-Tactin has to be taken into account for two reasons when recombinant proteins secreted to the cell culture supernatant are aimed to be directly purified via Strep-Tactin affinity chromatography:
  • It efficiently competes with the (Twin-)Strep-tag target proteinthereby preventing it from getting bound to the resin
  • it inactivates Strep-Tactin resins and thus make a re-use impossible.
BioLock is a ready-to-use and cost effective solution for the removal of free biotin from cell culture supernatant. It simply has to be added to the cell culture supernatant prior to purification.
The required amount of BioLock for effective biotin blocking can easily be determined since 1ml BioLock blocks 70 μg biotin. In addition we provide a table with the required BioLock amounts for standard mammalian and insect cell culture media. More information (pdf 1.1MB)

For detailed product information please download the data sheet.

For additional information contact info@stratech.co.uk.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.

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