Tuesday, September 30, 2014

Product Focus: HR Targeting Vectors for CRISPR/Cas9 and TALE-based Editing

No freezing or cooling during storage, shipping and handling!

Jena Bisocience’s Freeze-dried (lyophilized) PCR mixes are stable at ambient temperatures up to 30°C and allow point-of-care usage without any refrigerating infrastructure.
Vials containing the freeze-dried pellet are simply filled up with template DNA, primers and PCR-grade water, and the PCR can be run as usual. Lyophilized PCR mixes are available in single PCR tubes, 8-well tube strips, 96-well plates or custom-specific formats. In addition, we offer adaption to individual applications such as addition of primers or positive control DNA.
Jena Bioscience’s lyophilized PCR mixes come in a number of formats including:

Amplification directly from whole blood or tissues

  • avoids any prior DNA purification
  • minimizes amount of source material

Amplification of multiple fragments

  • optimized for multi-parallel target gene amplification in a single tube

Real-Time Master Lyophilisates

  • sample DNA quantification in broad dynamic range
  • exceptional sensitivity and selectivity

Taq and Hot Start Master Lyophilisates

  • robust, reliable results for all routine PCR
  • low background with hot start master mixes

Reverse Transcription Master Lyophilisates

  • engineered reverse transcriptase with enhanced thermal stability
  • increased specificity, high yield and efficiency for “difficult” (highly structured) cDNA fragments



Ready-to-Use Lyophilisates containing custom primers are custom made master mix lyophilisates comprising primers, enzyme, dNTPs and reaction buffer. Provide us with primer sequence and we deliver preloaded PCR tubes and plates ready-made for your special application. The only thing you still have to do is adding template RNA, filling up with water and starting the cycler!


Amplification Directly from Whole Blood or Tissues

Direct PCR Lyophilisate – Lyophilized Master Mix for direct PCR amplification from blood, animal and plant tissue
Direct PCR Lyophilisate is designed for PCR amplification directly from whole blood, animal tissues and plant tissues without the need of prior DNA purification processes. The robust enzyme in combination with a specially optimized unique buffer system resists various PCR inhibitors of blood and tissue samples.

Multiplex PCR Master Lyophilisate – Lyophilized Master Mix for multiplex PCR application
Multiplex PCR Master Lyophilisate is specially designed for the set-up of multiplex PCR reactions. It contains an optimized composition of polymerase, nucleotides, MgCl2 and stabilizing components in a specifically developed buffer system allowing the parallel amplification of a multitude of fragments in a single PCR assay.

Amplification of Multiple Fragments

Direct PCR Lyophilisate – Lyophilized Master Mix for direct PCR amplification from blood, animal and plant tissues
Direct PCR Lyophilisate is designed for PCR amplification directly from whole blood, animal tissues and plant tissues without the need of prior DNA purification processes. The robust enzyme in combination with a specially optimized unique buffer system resists various PCR inhibitors of blood and tissue samples.

Multiplex PCR Master Lyophilisate - Lyophilized Master Mix for multiplex PCR application
Multiplex PCR Master Lyophilisate is specially designed for the set-up of multiplex PCR reactions. It contains an optimized composition of polymerase, nucleotides, MgCl2 and stabilizing components in a specifically developed buffer system allowing the parallel amplification of a multitude of fragments in a single PCR assay.


Real-Time Master Lyophilisates

qPCR ProbesMaster Lyophilisate – Lyophilized real-time PCR Master Mix for dual labeled probes  
qPCR ProbesMaster Lyophilisate is designed for quantitative real-time analysis of DNA samples using DNA probe based detection. The lyophilisate is recommended for use with Dual Labeled Fluorescent Probes, e.g. TaqMan®, Molecular Beacons or FRET probes.
The Master contains all reagents required for qPCR (except template, primer and labeled fluorescent probe).

qPCR GreenMaster Lyophilisate – Lyophilized real-time PCR Master Mix with green-fluorescent DNA stain
qPCR GreenMaster Lyophilisate is designed for quantitative real-time analysis of DNA samples using the fluorescent DNA stain EvaGreen®. The fluorescent dye intercalates into the amplification product during the PCR process and enables the rapid analysis of target DNA without the need of sequence-specific labeled probes.
The Master contains all reagents required for qPCR (except template and primer).


Taq and Hot Start Master Lyophilisates

Red Load Taq Master Lyophilisate – Lyophilized Taq Master Mix containing red gel loading dye
Red Load Taq Master Lyophilisate contains additionally an inherent red dye allowing the direct loading of the PCR reaction product onto the gel.
To perform PCR, fill up the vials with a premix of primers and PCR-grade water and add DNA template. If necessary, centrifuge to remove bubbles, vortex the vials to assure homogeneity and start cycling.

Taq Master Lyophilisate – Lyophilized Taq Master Mix
Taq Master Lyophilisate combines highest performance with convenience of use and stability.
To perform PCR, fill up the vials with a premix of primers and PCR-grade water and add DNA template. If necessary, centrifuge to remove bubbles, vortex the vials to assure homogeneity and start cycling.

Hot Start Master Lyophilisate – Lyophilized Hot Start Master Mix
Hot Start Master Lyophilisate provides improved specificity and sensitivity when amplifying low-copy-number targets in complex backgrounds. The polymerase activity is blocked at ambient temperature and switched on automatically at the onset of the initial denaturation.


Reverse Transcription Master Lyophilisates

One-Step RT-PCR Master Lyophilisate – Lyophilized One-Step RT-PCR Master Mix
One-Step RT-PCR Master Lyophilisate is designed for performing maximum sensitive and specific RT-PCRs convenient in single tubes. The enzyme mix is based on a genetically engineered RT polymerase with enhanced thermal stability resulting in an increased specificity, higher cDNA yield and an improved efficiency for highly structured and long cDNA fragments.
The lyophilisate contains all reagents required for RT-PCR (except template and primer) in one tube to ensure fast and easy preparation with a minimum of pipetting steps. The premium quality enzyme mix, ultrapure dNTPs and the optimized complete reaction buffer ensure superior amplification results.

RT Master Lyophilisate – Lyophilized Master Mix for Reverse Transcription
RT Master Lyophilisate contains a genetically engineered version of M-MLV Reverse Transcriptase (M-MLV RT) with eliminated RNase H activity and increased thermal stability. The enzyme is a RNA-directed DNA polymerase that synthesizes a complementary DNA strand initiating from a primer using single-stranded RNA or DNA as template.
Each vial contains all components (except primers and template) required for a 20 µl reverse transcription assay. To perform the assay, only fill up the vials with a mix of primers and RNA template.



Where can I find more information about Jena Bioscience?

Visit the manufacturer page at www.stratech.co.uk/jena_bioscience, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.
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