Monday, March 16, 2015

NEW Products: aCella™ – TOX: Bioluminescence Non Radioactive Cytotoxicity Assay (GAPDH)

Key Benefits

  • Safe – Non radioactive enzyme release assay
  • Versatile – Assay can be run in serum-supplemented media
  • Homogenous – One-step, no wash assay which can be run in same plate as samples
  • FAST – Results in 3-5 minutes
  • Highly Sensitive – can detect less than 500 cells/well in 10% FCS
  • Adaptable for High Throughput screening
  • Non-destructive assay allows monitoring of additional parameters.
  • Perfect for ADCC, CMC & Complement Cytotoxicity assays

Description

Cell Technology offers aCella-TOX, a highly sensitive assay using its patented coupled luminescent technology for the detection of cytotoxicity. This assay is designed to quantitatively measure the release of Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) from mammalian cell lines or bacterial cells (1,2,3,4). Other enzyme release assays suffer from low sensitivity as a result of interference by serum or phenol red present in the media (i.e. Lactate Dehydrogenase (LDH release assay (5,6,7,8)).

The release of GAPDH is coupled with the enzyme 3-Phosphoglyeric Phosphokinase (PGK) to produce ATP. ATP is detected with aCella-TOX as a homogeneous assay that can measure cytoxicity or cell viability via the Luciferase-Luciferin Bioluminescence methodology. It is also possible to set up kinetic assays by transferring culture supernatants from the original assay plate onto a different plate. Additional parameters such as gene expression can also be monitored as the assay system is non-destructive.






Fig A: A Direct comparison of 51Cr and aCella-TOX was carried out with the same donors with Daudi Cells. Log (EC50) value for aCella-Tox was -2.23, and that for 51Cr was -2.087 for Donor A.


 

Fig B: A Direct comparison of 51Cr and aCella-TOX was carried out with the same donors with Daudi Cells. Log (EC50) value for aCella-TOX was -2.612, and that for 51Cr was -2.77 for Donor B.



Where can I find more information about Cell Technology?

Visit the manufacturer page at www.stratech.co.uk/cell_technology, email info@stratech.co.uk or call  +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.
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