Wednesday, April 22, 2015

NEW Product: AAVanced™ Concentration Reagent – One-step rAAV particle isolationsys

Easiest method for rAAV isolation
  • Easy to use, single reagent
  • Isolate rAAV directly from media
  • No cell lysates or centrifugation
  • Saves time and cost-effective
  • Compatible with all serotypes


AAVanced™ Concentration Reagent

One-step rAAV particle isolation from packaging cell media
Traditional rAAV production methods requires multiple steps, including cell lysis and CsCl2 ultra-high-speed density gradient centrifugation, chromatography, or binding to affinity matrix columns. These are difficult to setup, time-consuming, and require specialized equipment for isolation of high-purity rAAV for in vivo experiments. As many studies have shown that different rAAV serotypes are efficiently secreted into the culture medium of transfected 293T cells during rAAV packaging, SBI has developed a novel reagent to facilitate fast, high-quality rAAV isolation.

   

 

Easiest Protocol to Isolate rAAV Particles

SBI has developed an innovative solution called AAVanced Concentration Reagent – a simple, one step rAAV concentration reagent for the isolation of rAAV particles from media. AAVanced Concentration Reagent is based on a proven nanoparticle technology and is specifically optimized for the precipitation of most AAV serotypes from culture medium. The rAAV virus produced using AAVanced Concentration Reagent has been tested successfully for in vitro and in vivo applications for multiple serotypes of AAV with no observed cytotoxic effects, which provides direct evidence for the utility of this reagent for demanding applications using rAAV. SBI’s AAVanced Concentration Reagent significantly reduces the complexity and time required for rAAV virus production, allowing researchers to focus on their research experiments rather than virus production.

AAVanced Concentration Reagent Produces Robust Virions

How does AAVance virus preps compare to tranditonal cell lysate preps?
To test this comparison, recombinant rAAV was packaged with a PGK-GFP expression shuttle vector. The rAAV particles were isolated either by the traditional cell lysis freeze-thaw with centifugation method or with the AAVanced Concentration Reagent from the packaging cell media. Equal amounts of rAAV were then added to HEK293 cells to test for infectivity based on GFP expression. Representative images of GFP expressing cells 10 days after infection.

AAVanced virus produces infectious virus

AAVanced Concentration Reagent

AAVanced Concentration Reagent (100 ml) 100 ml AAV100A-1
AAVanced Concentration Reagent (250 ml) 250 ml AAV110A-1

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Where can I find more information about SBI?

Visit the manufacturer page at www.stratech.co.uk/SBI, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.
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