Tuesday, May 19, 2015

Product Focus: Microplate Based Snapshot ChIP Assay

Different gene expression depends not only on the binding of specific transcription factors (TFs) to discrete promoter elements but also on expression and interaction of co-activators with its associated TFs. The interactive cofactor complexes allow the functional integration of multiple TFs and pathways facilitating the formation of distinct biological programs. Unlike electrophoretic mobility shift assays detecting the binding of specific TFs present in cell lysates to DNA sequences in vitro, chromatin immunoprecipitation (ChIP) assay is enable the analysis of the association of a specific TF or co-factor with specific promoters in vivo and provides a snapshot of how a regulatory TF or co-factor affects the expression of a single gene or a variety of genes at the same time.
To simplify the ChIP assay in a high throughput method, Signosis has developed a 96-well microplate-based ChIP assay, where all of steps from IP to DNA elution are handled in microplate wells without sample transfers in an high throughput way. The assay is an ideal tool for studying up to 96 protein-DNA bindings in one sample or one protein-DNA binding in up to 96 samples, or multiple protein-DNA bindings in multiple samples/treatments.

Benefits:
  • High Throughput – 96 ChIP experiments can be performed simultaneously in one microplate.
  • Simple Sample Handling – All steps from IP to DNA elution are done in a single well, including crosslinking and fragmentation, without the need to transfer samples.
  • Reproducible and Time Saving – Plate-based ChIP assay eliminates errors introduced from conventional bead-based individual precipitation, washing and elution steps.
  • Affordable – More economical per reaction than conventional ChIP assay.


Principle

ChIP assays reveal complexity for chromatin, transcriptional regulation, RNA processing and DNA replication. These processes are regulated by multiple histone modifications that can be mediated by kinases, methyltransferases, acetyltransferase, ubiquitintransferase and others. In addition, hundreds of different RNA processing and transcription factors are involved in these epigenetic changes. As a result, the regulatory modifications of DNA-bound and accessory factors may be far greater in number and more complex than previously reported. Therefore, the ability to simultaneously detect multiple modification events will enhance our understanding of nuclear processes. The conventional ChIP method has notable limitations, including time-consuming procedure, bead-based individual IP and multiple tube transfers. These limitations result in potential sample loss, cross contamination and variability. It becomes a bigger problem when multiple chromatin samples are involved. Signosis provides a microplate-based chromatin ChIP assay kit, which allows monitoring the nuclear events in a high throughput way. The kit is optimized to perform all of IP steps from capturing the protein/DNA complexes to eluting DNA in one single well.


 


 User Manuals and Product Information

Microplate Snapshot ChIP Assay CP-0001

Oct4 Microplate Snapshot ChIP Assay CP-0101
NRF2 Microplate Snapshot ChIP Assay CP-0102

HDAC1 Microplate Snapshot ChIP Assay CP-0103
Smad Microplate Snapshot ChIP Assay CP-0104

Stat3 Microplate Snapshot ChIP Assay CP-0105
NFkB Microplate Snapshot ChIP Assay CP-0106
HIF Microplate Snapshot ChIP Assay CP-0107
H3 Microplate Snapshot ChIP Assay CP-0108
p300 Microplate Snapshot ChIP Assay CP-0109
p53 Microplate Snapshot ChIP Assay CP-0110


Where can I find more information about Signosis?

Visit the manufacturer page at www.stratech.co.uk/signosis, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.


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