Thursday, June 18, 2015

Product Focus: TUNEL Apo-Green Detection Kit

DNA fragmentation represents a characteristic of late stage apoptosis. DNA fragmentation in apoptotic cells can be detected by terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL).

Biotool™ TUNEL Apo-Green Detection Kit is a modified FITC-12-dUTP TUNEL Assay designed to provide simple, accurate and rapid detection of apoptotic cells. It measures nuclear DNA fragmentation, an important biochemical indicator of apoptosis by utilizing FITC-12-dUTP.

FITC-12-dUTP is more readily incorporated into DNA strand breaks than other larger ligands, giving rise to brighter signal that is sensitive and more accurate than traditional ligands. It also can be used to assay apoptotic cell death in both tissue sections and cultured cells.

 


Components List

Description B31112-BIT (20 rxns) B31115-BIT (50 rxns) B31118-BIT (100 rxns)
5 × Equilibration Buffer 1.25 ml 1.25 ml × 2 1.25 ml × 3
Apo-Green Labeling Mix 100 μl 250 μl 250 μl × 2
Recombinant TdT Enzyme 20 μl 50 μl 50 μl × 2
Proteinase K (2 mg/ml) 40 μl 100 μl 100 μl × 2
  



Where can I find more information about Biotool?

Visit the manufacturer page at www.stratech.co.uk/biotool, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.


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