Wednesday, July 1, 2015

Supplier Focus: Biotool – cDNA Synthesis SuperMix

Biotool offers a range of optimized cDNA synthesis systems (SuperMix) to generate cDNA for a variety of research interests.

All-in-One cDNA Synthesis SuperMix


BioScript All-in-One cDNA Synthesis SuperMix provides first-strand cDNA synthesis, optimized with the high-temperature capability of Reverse Transcriptase and an optimized SuperMix formula. In one tube, the pre-blended 5× qRT SuperMix contains all the necessary components for reverse transcription of YOUR RNA template. The resulting cDNA product is suitable for use in real-time quantitative RT-PCR (qRT-PCR).


  • Reproducibility: Modified Oligo dT: Random primers ratio facilitates qPCR repeatability.
  • High compatibility: cDNA product is suitable for application with SYBR Green or Probe qPCR.
 


 

SuperFast cDNA Synthesis SuperMix


SuperFast cDNA Synthesis SuperMix is designed for cDNA synthesis by genetic modification. Reverse transcription can be completed within 5 minutes, due to high affinity with RNA templates and a fast extension rate. The cDNA is efficiently synthesized by incubating the SuperFast RT/RI Enzyme Mix with the optimized 2 × TS SuperFast Reaction Mix. The cDNA products are suitable for downstream PCR and qPCR applications.


  • Fast synthesis: Only 5 minutes needed for reverse transcription.
  • Higher yield: Save RNA from degradation due to the fastest reverse transcription.
  • Size of cDNA: Up to 12 kb in product length, more full-length cDNA is obtained.

 


 

SuperZyme III cDNA Synthesis SuperMix


SuperZyme III cDNA Synthesis SuperMix provides all the necessary components for cDNA synthesis from total RNA or mRNA. The cDNA is efficiently synthesized by employing SuperZyme III RT/RI Enzyme Mix and an optimized 2×TS III Reaction Mix. RNase H-Deficiency reduces RNA template degradation during the 1st-strand cDNA synthesis. Product lengths of cDNA can be up to 15 kb. The cDNA products are suitable for downstream PCR, qPCR and RNA-sequencing applications.


 



ThermoStable cDNA Synthesis SuperMix


ThermoStable cDNA Synthesis SuperMix (+gDNA Remover) is engineered to have high thermal stability (up to 65°C) for efficient transcription of RNA regions with high secondary structure. It is stable over a broad range of reaction temperatures (42°C-65°C) and can achieve simultaneous genomic DNA (gDNA) removal and cDNA synthesis in one tube. This product generates the highest yields of cDNA, and is able to synthesize very long RNA transcripts up to 20 kb. The cDNA products are suitable for downstream PCR, qPCR and RNA-Sequencing applications.


High thermostability: ThermoStable Reverse Tramscriptase is active up to 65°C.
Double advantage: Simultaneous gDNA removal and cDNA synthesis.
Higher yield: Synthesize very long RNA transcripts up to 20 kb.

 



Where can I find more information about Biotool?

Visit the manufacturer page at www.stratech.co.uk/biotool, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.

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