Wednesday, July 1, 2015

Supplier Focus: Biotool – Hot Start High-Fidelity DNA Polymerase

Universe Hot Start High-Fidelity DNA polymerase

User GuideMSDS

Universe High-Fidelity Hot Start DNA polymerase is a superior enzyme in this category for robust PCR with extreme fidelity, featuring 53x error rate lower than Taq polymerase and extension rate as fast as 1 sec / kb*. Universe High-Fidelity Hot Start DNA polymerase possesses 5´→ 3´polymerase activity, 3´ → 5´exonuclease activity and will generate blunt-ended products. The polymerase is also capable of amplifying long fragments such as 20kb genomic DNA and 40kb λDNA. Our suggested applications include cloning, sequencing, genomic DNA amplification and high throughput PCR, etc.

Advantages

  • Robust: Maximal success with minimal optimization
  • Extreme Fidelity: 53x error rate lower than Taq
  • High Yield: Increased product yield using minimal enzyme
  • Versatile: Used for routine PCR, or with long, difficult templates

 

Materials Provided

Component B21101 100U (100 runs) B21102 250U (250 runs) B21103 500U (500 runs)
Universe High-Fidelity Hot Start DNA polymerase (1 U /μl) 100 μL 250 μL 500 μL
2 x Universe buffer 1.25 mL x 2 1.25 mL x 5 1.25 mL x 10
dNTP Mix (10mM each) 100 μL 250 μL 500 μL
10 x Loading buffer 1.25 mL 1.25 mL x 3 1.25 mL x 5

 

Universe Hot Start High-Fidelity 2x PCR Master Mix

Universe Hot Start High-Fidelity 2x PCR Master Mix

Biotool Universe Hot Start High-Fidelity 2x PCR Master Mix is a convenient premix version of Universe High-Fidelity Hot Start DNA Polymerase, which is a superior enzyme in this category for robust PCR with extreme fidelity, featuring 53x error rate lower than Taq polymerase and extension rate as fast as 1 sec / kb*. Universe High-Fidelity Hot Start DNA Polymerase possesses 5´→ 3´polymerase activity, 3´ → 5´exonuclease activity and will generate blunt-ended products. The polymerase is also capable of amplifying long fragments such as 20kb genomic DNA and 40kb λDNA. Our suggested applications include cloning, sequencing, genomic DNA amplification and high throughput PCR, etc.

* The extension rate varies with individual application.

Advantages

  • Robust: Maximal success with minimal optimization
  • Extreme Fidelity: 53x error rate lower than Taq
  • High Yield: Increased product yield using minimal enzyme
  • Versatile: Used for routine PCR, or with long, difficult templates
  • Convenient: Premix allows rapid reaction set-up

 

Materials Provided


Component B22101 (100 rxns) B22102 (250 rxns) B22103 (500 rxns)
Universe Hot Start High-Fidelity 2x PCR Master Mix 1.25mL x 2 1.25mL x 5 1.25mL x 10

 

Amplification Chart


Fig1: Amplification of genomic DNA templates of various lengths(0.6kB, 1.0kb, 2.6kb, 3.0kb, 4.0kb, 5.1kb, 6.2kb, 7.1kb, 8.5kb, 10.6kb, 17.8kb, 20.3kb, 21.4kb) using Universe High-Fidelity Hot Start DNA polymerase at extension rate of 15 seconds/kb.

Fig2: Amplification of λDNA and human genome DNA templates at various extension rate of 1sec/kb, 2 sec/kb, 5 sec/kb, 10 sec/kb, 15 sec/kb, 30 sec/kb, 60 sec/kb, respectively.



Where can I find more information about Biotool?

Visit the manufacturer page at www.stratech.co.uk/biotool, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.

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